That is possible - how much above 250 V? If only a few volts (say to 260 V) then damage is unlikely. If you mean 415 V, then you might easily have damaged both the emonTx and the a.c. adapter.

Definitely not. Doing that is part of the standard 3-phase calibration procedure.

Both these things point to either a power supply problem or the processor has failed or the sketch isn’t running.

The power is the first thing to check. When you had the 5 V USB supply connected, did you remove the link? Did you put it back when using only the a.c. adapter?
Did anything happen (LEDs light) when you connected the programmer and 5 V from the computer?

Do you have (or beg, borrow?) a multimeter that you can use to measure voltages?

Can you measure the a.c. voltage from your a.c. adapter? It should be around 12 V.
With the programmer & computer only connected, do you measure 5 V d.c between screw terminals 1 (5 V) & 3 (GND).
With the 5 V USB power supply only connected, do you measure 5 V d.c. between screw terminals 1 (5 V) & 3 (GND).

If you have 5 V d.c. in both cases, with the 5 V present and the jumper link removed, do you measure 3.3 V d.c. between screw terminals 2 (3.3 V) & 3 (GND).

With the a.c. adapter connected and the link removed, do you measure 3.3 V d.c. between the side of the jumper link furthest from the screw terminals and GND?

When you replace the jumper link, and with no 5 V present, do you still measure 3.3 V d.c. between screw terminals 2 (3.3 V) & 3 (GND).

If you don’t have 3.3 V, then the emonTx may well be damaged beyond repair, but The Shop will need to confirm that.

If you do have 3.3 V, can you load a sketch - any sketch? The standard Arduino “Blink” is the simplest, though you must change LED_BUILTIN to [DIO] 6.

If you cannot load a sketch, then either the bootloader has been damaged, or the processor itself is damaged. It’s possible to install a bootloader, but the FTDI programmer won’t do it, you need either an ICSP programmer or an Arduino.

If the processor is damaged, then the emonTx is almost certainly beyond repair.

Let us know the results so far.